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Test 6: Bacterial Growth and Reproduction Multiple Choice (choose one answer)

1. In bacterial cultures, growth can be demonstrated by an increase in:

A.mass B.cell size C.cell number D.cell length

2. DNA replication in bacteria is controlled by:

A.cell size B.cell division C.cell separation D.cell initiation

3. During which phase of bacterial growth is there an increase in cell size but not in cell number?

A.lag B.log C.stationary D.exponential

4. The generation time for bacteria is determined by:

A.measuring the time it takes to double the number of bacteria from the time the culture was initiated until the beginning of stationary phase

B.measuring the time it takes to double the number of bacteria from lag phase to death phase

C.measuring the time it takes to double the number of bacteria from log phase to the end of stationary phase

D.measuring the time it takes to double the number of bacteria from log phase to the beginning of stationary phase

5. Most pathogenic bacteria are considered:

A.psychrophiles B.mesophiles C.thermophiles D.merophiles

6. Bacteria that grow at low nutrient concentrations are referred to as:

A.autotrophsB.phototrophs C.copiotrophs D.oligotrophs

7. In times of nutrient deficiencies, the bacteria Clostridium produce____until conditions are permissive for vegetative growth.

A.prosthecae B.spores C.stalks D.fruiting bodies

8. The temperature of the incubator was raised from 15~(2 to 35~(2. The cultures in the incubator demonstrated a____fold increase in enzymatic activity.

A.two B.four C.eight D.twenty

9. Organisms that grow at or near their optimal growth temperature are called:

A.stenothermal bacteria B.euthermal bacteria C.cauldoactive bacteria D.mesophilic bacteria

10. All of the following are toxic oxygen products except:

A.02 B.OH- C.H20 D..H202

11. Catalase, which is produced by Staphylococci, catalyzes which of the following reactions? A.202+ 2H+ →2H202 + 02    B.2H202→2H20 + 02

C.H202 + NADH + H+→2H20 + NAD    D.H202 + e- + H+→H2O + OH-

12. A saturated solution of NaC1 has a water activity index of:

A.1.0 B.0.90 C.0.80 D.0.70

13. Organisms that can grow at a water index at or below that of NaCI are called:

A.xerotolerant B.salt tolerant C.mesotolerant D.salotolerant

14. All of the following organisms will survive an environment of 0.9 Aw except:

A.Lactobacillus B.Staphylococcus C.Saccharomyces D.SpiriUum

15. Halophiles are classified as organisms that require ____for growth.

A.sugar B.salt C.water D.air

16. Osmophiles require a ____Aw for growth.

 A.low B.high

17. The pressure exerted on a cell due to high solute concentrations is referred to as:

A.osmotic pressure B.hydrostatic pressure C.barometric pressure D.surface tension

18. A diver encountered a new bacterial isolate while she was diving at 1000 m. The organism will be classified as:

A.marine B.barotolerant C.barophilic D.normal

19. Fungi can be differentiated from most bacteria by culturing at:

A.marine B.low pH C.neutral pH

20. All phototacfic bacteria respond to light by:

A.moving away from the source of light B.moving toward the source of light C.increasing the movement of their flagella D.creating gas vesicles to rise to the surface

Fill in the Blank

1. Organisms that grow best above 40℃ are called______.

2. Organisms that grow best below 20℃ are called______.

3. Organisms that grow best between 20 and 40℃ are called ______.

4. Myxobacteria form unique structures called ______ to cope with nutrient deficiencies.

5. Bacteria that grow only at reduced oxygen concentrations are called _______.

6. Bacteria that require oxygen for growth are called_____.

7. Bacteria that grow at high nutrient concentrations are called ______.

8. Caulobacter is an example of a ______ bacteria.

9.At temperatures above the optimum, E. coli and other bacteria induce a change in gene expression called______.

10. A change in hydrostatic pressure of 10 atm is experience with an increase in depth of ______ m. Matching Cell Cycle

 Matching: l. C a. cell enlargement 2. M b. condensation of chromosomes 3. G1 c. replication of the genome 4. G2 d. separation of chromosomes 5. S e. cell division